Publications

A drawing outlining the diffusion of H2) and CO2 inside of a leaf.

A guide to photosynthetic gas exchange measurements: Fundamental principles, best practice and potential pitfalls

Florian Busch, Lisa Ainsworth, Anna Amtmann, Amanda Cavanagh, Steven Driever, John Ferguson, Johannes Kromdijk, Tracy Lawson, Andrew Leakey, Jack Matthews, Katherine Meacham-Hensold, Richard Vath, Silvere Vialet-Chabrand, Berkley Walker, Maria Papanatsiou

Plant, Cell & Environment

Young Cho kneels surrounded by plants in a field trial

Reducing chlorophyll levels in seed-filling stages results in higher seed nitrogen without impacting canopy carbon assimilation

Young B. Cho, Ryan A. Boyd, Yudong Ren, Moon-Sub Lee, Sarah I. Jones, Ursula M. Ruiz-Vera, Justin M. McGrath, Michael D. Masters, Donald R. Ort

Plant, Cell & Environment

Sunlight filtering through upper layers of the corn canopy.

Is chloroplast size optimal for photosynthetic efficiency?

Katarzyna Glowacka, Johannes Kromdijk, Coralie Salesse-Smith, Caitlin Smith, Steven M. Driever, Stephen Long

New Phytologist

Seven green soybean pods hang on a stem.

Impact of pod and seed photosynthesis on seed filling and canopy carbon gain in soybean

Young B Cho, Samantha Stuttz, Sarah I Jones, Yu Wang, Elena A Pelech, & Don Ort

Plant Physiology

A diagram of the stomatal opening of a plant.

Calvin cycle and guard cell metabolism impact stomatal function

Pauline Lemonnier, Tracy Lawson

Seminars in Cell and Developmental Biology

Soybean leaves in circles around a bright yellow light.

Escherichia coli expressing chloroplast chaperones as a proxy to test heterologous Rubisco production in leaves

Sally Buck, Tim Rhodes, Matteo Gionfriddo, Tanya Skinner, Ding Yuan, Rosemary Birch, Maxim V Kapralov, Spencer M Whitney

Journal of Experimental Botany

Maize

Increased bundle-sheath leakiness of CO2 during photosynthetic induction shows a lack of coordination between the C4 and C3 cycles

Yu Wang, Samantha S. Stutz, Carl J. Bernacchi, Ryan A. Boyd, Donald R. Ort

New Phytologist

Danny Gotarkar

High-throughput Analysis of Non-Photochemical Quenching in Crops using Pulse Amplitude Modulated Chlorophyll Fluorometry

Dhananjay Gotarkar, Lynn Doran, Meghan Burns, Abigail Hinkle, Johannes Kromdijk, Steven J. Burgess

JoVE

Science Cover: Soybean photosynthesis and crop yield are improved by accelerating recovery from photoprotection

Soybean photosynthesis and crop yield are improved by accelerating recovery from photoprotection

Amanda P. De Souza, Steven J. Burgess, Lynn Doran, Jeffrey Hansen, Lusya Manukyan, Nina Maryn, Dhananjay Gotarkar, Lauribeth Leonelli, Krishna K. Niyogi, Stephen P. Long

Science

Arabidopsis Hexokinase 1_thumbnail

Guard-cell-targeted overexpression of Arabidopsis Hexokinase 1 can improve water use efficiency in field-grown tobacco plants

Liana G Acevedo-Siaca, Katarzyna Głowacka, Steven M Driever, Coralie E Salesse-Smith,Nitsan Lugassi, David Granot, Stephen P Long, Johannes Kromdijk

Journal of Experimental Botany

ictB transformants_thumbnail

Field-grown ictB tobacco transformants show no difference in photosynthetic efficiency for biomass relative to wildtype

Ursula M. Ruiz-Vera, Liana G. Acevedo-Siaca, Kenny L. Brown, Chidi Afamefule, Hussein Gherli, Andrew J. Simkin, Stephen P. Long, Tracy Lawson, Christine Raines

Journal of Experimental Botany

Dr Tory Clarke

Mesophyll conductance is unaffected by expression of Arabidopsis PIP1 aquaporins in the plasmalemma of Nicotiana

Victoria C. Clarke, Annamaria De Rosa, Baxter Massey, Aleu Mani George, John R. Evans, Susanne von Caemmerer, Michael Groszmann

Journal of Experimental Botany

Cowpea thumbnail

Cowpea leaf width correlates with above ground biomass across diverse environments

Anthony Digrado, Emmanuel Gonzalez-Escobar, Nicole Owston, Rhiannon Page, Saba Baba Mohammed, Muhammad Lawan Umar, Ousmane Boukar, Elizabeth A. Ainsworth, Elizabete Carmo-Silva

Legume Science

A general overview of remote and proximal sensing techniques used for HTP of photosynthesis.

Advances in field-based high-throughput photosynthetic phenotyping

Peng Fu, Christopher M Montes, Matthew H Siebers, Nuria Gomez-Casanovas, Justin M McGrath, Elizabeth A Ainsworth, Carl J Bernacchi

Journal of Experimental Botany

Cowpea

Faster than expected Rubisco deactivation in shade reduces cowpea photosynthetic potential in variable light conditions

Samuel H. Taylor, Emmanuel Gonzalez-Escobar, Rhiannon Page, Martin A. J. Parry, Stephen P. Long, Elizabete Carmo-Silva

Nature Plants

Overhead shot of heaters in the fields

Alternative pathway to photorespiration protects growth and productivity at elevated temperatures in a model crop

Amanda P. Cavanagh, Paul F. South, Carl J. Bernacchi, Donald R. Ort

The Plant Biotechnology Journal

Peng Fu Drought Imprints_thumbnail

Drought imprints on crops can reduce yield loss: Nature's insights for food security

Peng Fu, Deepak Jaiswal, Justin M. McGrath, Shaowen Wang, Stephen P. Long, Carl J. Bernacchi

Food and Energy Security

Dynamic Photosynthesis Model_thumbnail

Towards a dynamic photosynthesis model to guide yield improvement in C4 crops

Yu Wang, Kher Xing Chan, Stephen P. Long

The Plant Journal

PNAS_Rubisco Proton

Rubisco proton production can drive the elevation of CO2 within condensates and carboxysomes

Benedict M. Long, Britta Förster, Sacha B. Pulsford, G. Dean Price, Murray R. Badger

Proceedings of the National Academy of Sciences

Figure 1

The effect of increasing temperature on crop photosynthesis: from enzymes to ecosystems.

Caitlin E. Moore, Katherine Meacham-Hensold, Pauline Lemonnier, Rebecca A. Slattery, Claire Benjamin, Carl J. Bernacchi, Tracy Lawson, Amanda P. Cavanagh

Journal of Experimental Botany

Nature Plants figure

Technologies to deliver food and climate security through agriculture

Peter Horton, Stephen P. Long, Pete Smith, Stephen A. Banwart, David J. Beerling

Nature Plants

Photoprotection of the photosynthetic membrane.

Perspectives on improving light distribution and light use efficiency in crop canopies

Rebecca A. Slattery, Donald R. Ort

Plant Physiology

Flag Leaf

Variation between rice accessions in photosynthetic induction in flag leaves and underlying mechanisms

Liana G. Acevedo-Siaca, Robert Coe, W. Paul Quick, Stephen P. Long

Journal of Experimental Botany

SIF Yield Relationship

The inverse relationship between solar-induced fluorescence yield and photosynthetic capacity: benefits for field phenotyping

Peng Fu, Katherine Meacham-Hensold, Matthew H Siebers, Carl J Bernacchi

Journal of Experimental Botany

Cassava field trials (aerial image)

High sink strength prevents photosynthetic down-regulation in cassava grown at elevated CO2 concentration

Ursula M. Ruiz-Vera, Amanda P. De Souza, Michael R. Ament, Roslyn M. Gleadow, Donald R. Ort

Journal of Experimental Botany

Microscopy image of plant cells with bundle sheath cells identified.

Insights into the regulation of the expression pattern of Calvin-Benson-Bassham cycle enzymes in C3 and C4 grasses

Chidi Afamefule and Christine Raines

Frontiers of Plant Science

Table 1

During photosynthetic induction, biochemical and stomatal limitations differ between Brassica crops

Samuel H. Taylor, Douglas J. Orr, Elizabete Carmo-Silva, and Stephen P. Long

Plant, Cell & Environment

Cowpea rows

Assessing diversity in canopy architecture, photosynthesis, and water‐use efficiency in a cowpea magic population

Anthony Digrado, Noah G. Mitchell, Christopher M. Montes, Paulina Dirvanskyte, and Elizabeth A. Ainsworth

Food and Energy Security

Oxford interns and Pignon

Light, Not Age, Underlies the Maladaptation of Maize and Miscanthus Photosynthesis to Self-Shading

Robert F. Collison, Emma C. Raven, Charles P. Pignon, and Steven P. Long

frontiers in Plant Science

Aerial view of 2016 field trials

Stimulating photosynthetic processes increases productivity and water-use efficiency in the field

Patricia E. López-Calcagno, Kenny L. Brown, Andrew J. Simkin, Stuart J. Fisk, Silvere Vialet-Chabrand, Tracy Lawson, and Christine A. Raines

Nature Plants

Three authors pose next to wheat in the greenhouse.

An isoleucine residue improves Rubisco activase activity at high temperatures by 30%

Gustaf E. Degen, Dawn Worrall, and Elizabete Carmo-Silva

The Plant Journal

Figure from paper.

Plot-level rapid screening for photosynthetic parameters using proximal hyperspectral imaging

Katherine Meacham-Hensold, Peng Fu, Jin Wu, Shawn Serbin, Christopher M Montes, Elizabeth Ainsworth, Kaiyu Guan, Evan Dracup, Taylor Pederson, Steven Driever, and Carl Bernacchi

Journal of Experimental Botany

Image shows phenotypic differences in 8 rice varieties.

Variation in photosynthetic induction between rice accessions and its potential for improving productivity

Liana G. Acevedo‐Siaca, Robert Coe, Yu Wang, Johannes Kromdijk, W. Paul Quick, and Stephen P. Long

New Phytologist

A picture of the model's output.

Photosynthesis in the fleeting shadows: An overlooked opportunity for increasing crop productivity?

Yu Wang, Steven Burgess, Elsa de Becker, Stephen Long

Plant Journal

Figure 1 from paper.

Estimating photosynthetic traits from reflectance spectra: A synthesis of spectral indices, numerical inversion, and partial least square regression

Peng Fu, Katherine Meacham‐Hensold, Kaiyu Guan, Jin Wu, and Carl Bernacchi

Plant, Cell & Environment

Figure from paper.

Photosynthetic efficiency and mesophyll conductance are unaffected in Arabidopsis thaliana aquaporin knock-out lines

Johannes Kromdijk, Katarzyna Głowacka, and Stephen Long

Journal of Experimental Botany

Figure 3 from paper.

Genetic strategies for improving crop yields

Julia Bailey-Serres, Jane E. Parker, Elizabeth A. Ainsworth, Giles E. D. Oldroyd, and Julian I. Schroeder

Nature

Figure from paper.

Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady‐state, but by stomatal conductance in fluctuating light

Amanda P. De Souza, Yu Wang, Douglas J. Orr, Elizabete Carmo‐Silva, and Stephen P. Long

New Phytologist

Chlamydomonas reinhardtii

Thylakoid localized bestrophin-like proteins are essential for the CO2 concentrating mechanism of Chlamydomonas reinhardtii.

Ananya Mukherjee, Chun Lau, Charlotte Walker, Ashwani Rai, Camille Prejean, Gary Yates, Thomas Emrich-Mills, Spencer Lemoine, David Vinyard, Luke Mackinder, and James V. Moroney

Proceedings of the National Academy of Sciences

Thumbnail image of a figure from the paper

Hyperspectral leaf reflectance as proxy for photosynthetic capacities: An ensemble approach based on multiple machine learning algorithms

Peng Fu, Katherine Meacham-Hensold, Kaiyu Guan, and Carl J Bernacchi

Frontiers in Plant Science

Figure from paper.

High-throughput field phenotyping using hyperspectral reflectance and partial least squares regression (PLSR) reveals genetic modifications to photosynthetic capacity

Katherine Meacham-Hensold, Christopher Montes, Jin Wu, Kaiyu Guan, Peng Fu, Elizabeth Ainsworth, Taylor Pederson, Caitlin Moore, Kenny Brown, Christine Raines, and Carl Bernacchi

Remote Sensing of Environment

Figure from paper.

Carbon assimilation in crops at high temperatures

Rebecca A. Slattery and Donald R. Ort

Plant, Cell & Environment

Figure from paper.

Predicting light-induced stomatal movements based on the redox state of plastoquinone: Theory and validation

Johannes Kromdijk, Katarzyna Głowacka, and Stephen P. Long

Photosynthesis Research

Schematic representation of photosynthetic electron transport

Feeding the world: Improving photosynthetic efficiency for sustainable crop production

Andrew J. Simkin, Patricia E. López-Calcagno, and Christine A. Raines

Journal of Experimental Botany

Modified plant (left) beside unmodified plant (right)

Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field

Paul F. South, Amanda P. Cavanagh, Helen W. Liu, and Donald R. Ort

Science

Figure from paper

BSD2 is a Rubisco specific assembly chaperone, forms intermediary hetero‐oligomeric complexes and is non‐limiting to growth in tobacco

Brendon Conlan, Rosemary Birch, Celine Kelso, Sophie Holland, Amanda P. De Souza, Stephen P. Long, Jennifer L. Beck, and Spencer M. Whitney

Plant, Cell & Environment

carboxysome cover art

Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts

Benedict M. Long, Wei Yih Hee, Robert E. Sharwood, Benjamin D. Rae, Sarah Kaines, Yi-Leen Lim, Nghiem D. Nguyen, Baxter Massey, Soumi Bala, Susanne von Caemmerer, Murray R. Badger, and G. Dean Price

Nature Communications

Figure from paper

Overexpressing the H‐protein of the glycine cleavage system increases biomass yield in glasshouse and field grown transgenic tobacco plants

Patricia E. Lopez‐Calcagno, Stuart Fisk, Kenny L. Brown, Simon E. Bull, Paul F. South, and Christine A. Raines

Plant Biotechnology Journal

Figure

Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop

Katarzyna Głowacka, Johannes Kromdijk, Katherine Kucera, Jiayang Xie, Amanda P. Cavanagh, Lauriebeth Leonelli, Andrew D. B. Leakey, Donald R. Ort, Krishna K. Niyogi, and Stephen P. Long

Nature Communications

Figure from paper.

An improved Escherichia coli screen for Rubisco identifies a protein–protein interface that can enhance CO2-fixation kinetics

Robert H. Wilson, Elena Martin-Avila, Carly Conlan, and Spencer M. Whitney

Journal of Biological Chemistry

Figure from paper

Chlorophyll can be reduced in crop canopies with little penalty to photosynthesis

Berkley J. Walker, Darren T. Drewry, Rebecca A. Slattery, Andy VanLoocke, Young B. Cho, and Donald R. Ort

Plant Physiology

Figure from paper.

Identification and characterization of a solute carrier, CIA8, involved in inorganic carbon acclimation in Chlamydomonas reinhardtii

Marylou C. Machingura, Joanna Bajsa-Hirschel, Susan M. Laborde, Joshua B. Schwartzenburg, Bratati Mukherjee, Ananya Mukherjee, Steve V. Pollock, Britta örster, G. Dean Price, and James V. Moroney

Journal of Experimental Botany

Figure from paper.

Progress and challenges of engineering a biophysical CO2-concentrating mechanism into higher plants

Benjamin D. Rae, Benedict M. Long, Britta Förster, Nghiem D. Nguyen, Christos N. Velanis, Nicky Atkinson, Wei Yih Hee, Bratati Mukherjee, G. Dean Price, and Alistair J. McCormick

Journal of Experimental Botany

Figure from paper.

Arabidopsis CP12 mutants have reduced levels of phosphoribulokinase and impaired function of the Calvin–Benson cycle

Patricia López-Calcagno, Omar Abuzaid, Tracy Lawson, and Christine Raines

Journal of Experimental Biology

Figure from paper.

Photosynthesis, light use efficiency, and yield of reduced-chlorophyll soybean mutants in field conditions

Rebecca A. Slattery, Andy VanLoocke, Carl J. Bernacchi, Xin-Guang Zhu, and Donald R. Ort

Frontiers in Plant Science

figure

The many types of carbonic anhydrases in photosynthetic organisms

Robert J. DiMario, Marylou C. Machingura, Grover L. Waldrop, and James V. Moroney

Plant Science

Figure

Components of mesophyll resistance and their environmental responses: A theoretical modeling analysis

Yi Xiao and Xin-Guang Zhu

Plant, Cell & Environment

Figure

The impact of modifying photosystem antenna size on canopy photosynthetic efficiency

Qingfeng Song, Yu Wang, Mingnan Qu, Donald R. Ort, and Xinguang Zhu

Plant, Cell & Environment

Figure

Development of a three-dimensional ray-tracing model of sugarcane canopy photosynthesis and its application in assessing impacts of varied row spacing

Yu Wang, Qingfeng Song, Deepak Jaiswal, Amanda P. de Souza, Stephen P. Long, and Xin-Guang Zhu

BioEnergy Research

Figure

Measuring CO2 and HCO3 − permeabilities of isolated chloroplasts using a MIMS-18O approach

Dimitri Tolleter, Vincent Chochois, Richard Poiré, G. Dean Price, and Murray R. Badger

Journal of Experimental Botany

Figure

The role of sink strength and nitrogen availability in the down-regulation of photosynthetic capacity in field-grown Nicotiana tabacum L. at elevated CO2 concentration

Ursula M. Ruiz-Vera, Amanda P. De Souza, Stephen P. Long, and Donald R. Ort

Frontiers in Plant Science

Figure

Bile acid sodium symporter BASS6 can transport glycolate and is involved in photorespiratory metabolism in Arabidopsis thaliana

Paul F. South, Berkley J. Walker, Amanda P. Cavanagh, Vivien Rolland, Murray Badger, and Donald R. Ort

Plant Cell

Figure

Plant carbonic anhydrases: Structures, locations, evolution, and physiological roles

Robert J. DiMario, Harmony Clayton, Ananya Mukherjee, Martha Ludwig, and James V. Moroney

Molecular Plant

Figure from paper.

A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman, and James V. Moroney

Plant Methods

Figure from paper.

Uncertainty in measurements of the photorespiratory CO2 compensation point and its impact on models of leaf photosynthesis

Berkley J. Walker, Douglas J. Orr, Elizabete Carmo-Silva, Martin A. J. Parry, Carl J. Bernacchi, and Donald R. Ort

Photosynthesis Research

Science Magazine

Improving photosynthesis and crop productivity by accelerating recovery from photoprotection

Johannes Kromdijk, Katarzyna Głowacka, Lauriebeth Leonelli, Stéphane T. Gabilly, Masakazu Iwai, Krishna K. Niyogi, and Stephen P. Long

Science

Plants

Decreasing, not increasing, leaf area will raise crop yields under global atmospheric change

Venkatraman Srinivasan, Praveen Kumar, and Stephen P. Long

Global Change Biology

Figure

Rooting for cassava: insights into photosynthesis and associated physiology as a route to improve yield potential

Amanda P. De Souza, Lynnicia N. Massenburg, Deepak Jaiswal, Siyuan Cheng, Rachel Shekar, and Stephen P. Long

New Phytologist

Figure

Genetic and physiological diversity in the leaf photosynthetic capacity of soybean

Kazuma Sakodaa, Yu Tanaka, Stephen P. Long, and Tatsuhiko Shiraiwa

Crop Science

Figure

Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency

Douglas Orr, André Alcântara, Maxim Kapralov, John Andralojc, Elizabete Carmo-Silva, and Martin A. J. Parry

Plant Physiology

Figure

Light sheet microscopy reveals more gradual light attenuation in light-green versus dark-green soybean leaves

Rebecca A. Slattery, Aleel K. Grennan, Mayandi Sivaguru, Rosangela Sozzani, and Donald R. Ort

Journal of Experimental Botany

Figure

Physiological evidence for plasticity in glycolate/glycerate transport during photorespiration

Berkley J. Walker, Paul F. South, and Donald R. Ort

Photosynthesis Research

Figure

Cyanobacterial CO2-concentrating mechanism components: Function and prospects for plant metabolic engineering

Benedict M. Long, Benjamin D. Rae, Vivien Rolland, Britta Förster, and G. Dean Price

Current Opinion in Plant Biology

Figure

Regulation and levels of the thylakoid K+ /H+ Antiporter KEA3 shape the dynamic response of photosynthesis in fluctuating light

Ute Armbruster, Lauriebeth Leonelli, Viviana Correa Galvis, Deserah Strand, Erica Quinn, Martin Jonikas, and Krishna Niyogi

Plant and Cell Physiology

Figure

An improved approach for measuring the impact of multiple CO2 conductances on the apparent photorespiratory CO2 compensation point through slope–intercept regression

Berkley J. Walker, Dane C. Skabelund, Florian A. Busch, and Donald R. Ort

Plant, Cell & Environment

Figure

One crop breeding cycle from starvation? How engineering crop photosynthesis for rising CO2 and temperature could be one important route to alleviation

Johannes Kromdijk and Stephen P. Long

Proceedings of the Royal Society B Biological Sciences

Figure

Plants in silico: Why, why now and what?—an integrative platform for plant systems biology research

Xin-Guang Zhu, Jonathan P. Lynch, David S. LeBauer, Andrew J. Millar, Mark Stitt, and Stephen P. Long

Plant, Cell & Environment

Figure

An evaluation of new and established methods to determine T-DNA copy number and homozygosity in transgenic plants.

Katarzyna Głowacka, Johannes Kromdijk, Lauriebeth Leonelli, Krishna K. Niyogi, Tom E. Clemente, and Stephen P. Long

Plant, Cell & Environment

Figure

The costs of photorespiration to food production now and in the future

Berkley J. Walker, Andy VanLoocke, Carl J. Bernacchi, and Donald R. Ort

Annual Review of Plant Biology

Figure

Rubisco catalytic properties of wild and domesticated relatives provide scope for improving wheat photosynthesis

Anneke Prins, Douglas J. Orr, P. John Andralojc, Matthew P. Reynolds, Elizabete Carmo-Silva, and Martin A. J. Parry

Journal of Experimental Botany Advance Access

Figure from paper

Pharoah’s dream revisited: An integrated U.S. Midwest field research network for climate adaptation

David Gustafson, Michael Hayes, Emily Janssen, David B. Lobell, Stephen Long, Gerald C. Nelson, Himadri B. Pakrasi, Peter Raven, G. Philip Robertson, Richard Robertson, and Donald Wuebbles

BioScience

Figure from paper.

Redesigning photosynthesis to sustainably meet global food and bioenergy demand

Donald R. Ort, Sabeeha S. Merchant, Jean Alric, Alice Barkan, Robert E. Blankenship, Ralph Bock, Roberta Croce, et al.

PNAS

Figure

Meeting the global food demand of the future by engineering crop photosynthesis and yield potential

Stephen P. Long, A. Marshall-Colon, and X. G. Zhu

Cell

Plant Photosynthesis

Improving recombinant Rubisco biogenesis, plant photosynthesis and growth by coexpressing its ancillary RAF1 chaperone

Spencer M. Whitney, Rosemary Birch, Celine Kelso, Jennifer L. Beck, and Maxim V. Kapralov

PNAS

Simultaneous Improvement

Simultaneous improvement in productivity, water use, and albedo through crop structural modification

Darren T. Drewry, Praveen Kumar, and Stephen P. Long

Global Change Biology

Transplastomic Integration

Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts

J. J. L. Pengelly, B. Förster, S. von Caemmerer, M. R. Badger, G. D. Price, and S. M. Whitney

Journal of Experimental Botany

Photosynthesis

e-photosynthesis: a comprehensive dynamic mechanistic model of C3 photosynthesis from light capture to sucrose synthesis

Xin-Guang Zhu, Yu Wang, Donald R. Ort, and Stephen P. Long

Plant, Cell & Environment