Plant scientists at Lancaster University, with support from the University of Illinois, have made an important advance in understanding the natural diversity of a key plant enzyme which could help us address the looming threat of global food security.

Rubisco is the central enzyme responsible for photosynthesis in plants, it enables them to absorb carbon from the atmosphere, which they depend upon to live and grow. But until now, the extent of natural diversity of Rubisco across plant species was relatively unknown.

In the most comprehensive study of its kind to date, the Lancaster University-led research team has discovered that some of the species they looked at had more effective and high-performing Rubisco than several of the major crops species, like wheat and soybean. Their work was supported by RIPE (Realizing Increased Photosynthetic Efficiency), a research grant led by Illinois at the Carl R. Woese Institute for Genomic Biology.

As part of the study, researchers from Lancaster University and Liverpool John Moores University looked at 75 plant species, including grasses, wild rice, melons and beans from across the world and assessed the ability of their Rubiscos to assimilate CO2 at a range of different temperatures - to replicate the effects of a changing climate.

Publishing in the journal Plant Physiology, researchers say they were excited by the range of performance of Rubiscos isolated from the different land plants.

Some of these Rubisco enzymes have superior characteristics that now offer the possibility of engineering plants which grow more quickly, and with less need for additional fertilizers. As part of the consortium RIPE, the Lancaster researchers and their collaborators are working toward improving crops, including rice, cassava, soybean and cowpea.

Douglas Orr, a research associate at Lancaster University, said: "The plants we examined came from a range of environments, from sub-Saharan Africa to temperate regions of Europe and Asia, and northern Australia."

"We also analyzed the effect of temperature on Rubisco biochemistry in all these species, to explore how different Rubiscos respond to changing temperatures, which can help us understand how the changes occurring in our climate may impact plant growth."

"These discoveries are an important advance for RIPE," said RIPE Director Stephen Long, Professor of Plant Biology and Crop Sciences at the University of Illinois. "They show the existence of forms of this key enzyme that introduced into crops would increase their productivity and efficiency of both nitrogen and water use, and forms that will function well at future increased global temperatures."

"This large dataset has shed new light on the variation present in nature. We were able to identify a number of 'superior' Rubiscos which modeling suggests could improve photosynthetic efficiency in crops such as wheat and soybean. This provides important information in our efforts to produce more sustainable crops," said Elizabete Carmo-Silva, a lecturer at Lancaster University.

Professor Martin Parry, Lancaster University, said: "This new information gives us the opportunity to tailor the photosynthetic performance of crops for specific environments."

The study 'Surveying Rubisco diversity and temperature response to improve crop photosynthetic efficiency' is available online (doi: http://dx.doi.org/10.1104/pp.16.00750). RIPE is funded by the Bill & Melinda Gates Foundation. The Lancaster Environment Centre RIPE team is led by Professor Martin Parry and the overall RIPE project is led by Professor Steve Long (University of Illinois, who recently also joined Lancaster University).

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By: Beth Broomby || Lancaster University